A recently published article by ToxStrategies scientists presents the systematic identification, appraisal, and integration of mechanistic data in an assessment of the potential carcinogenicity of the non-nutritive sweetener acesulfame K (Ace K). Based on the evaluation of more than 800 measurements/assay endpoints related to one or more key characteristics of carcinogens (KCC) reported in the literature, and via high-throughput screening data, the authors found a lack of evidence for activity across the mechanistic database. These results align with the overall lack of tumor response to Ace K in mammalian carcinogenicity studies, corroborating previous reports of a lack of carcinogenicity related to Ace K exposure. This open-access publication is the third in a series of assessments for non-nutritive sweeteners, following aspartame and sucralose, all published in Food and Chemical Toxicology.