The Toxicity of Microplastics Explorer (ToMEx) was released as an open source, open access database and web application for microplastics toxicity three years ago for the purpose of data exploration, visualization, and analysis, providing an invaluable tool to the research community. Yet, the peer-reviewed literature has continued to grow exponentially, soon rendering ToMEx out of date. To ensure the continued utility of ToMEx, a crowd-sourcing approach was utilized to create a global workgroup to update ToMEx by extracting data from additional studies published since the original release. Through this process, both the aquatic and human health ToMEx databases roughly doubled in size. While a greater diversity of species and test particle characteristics are now represented in the aquatic organisms database, diversity within the human health database remains more limited. Select analyses previously conducted using the original database were repeated using the new, ToMEx 2.0 database to determine if new or additional trends or insights could be identified. Specifically, a previously developed framework for developing health-based microplastic thresholds for the state of California was applied using the newly updated database to determine if thresholds would be improved. However, data fit for the purpose of threshold development were still limited as they were for ToMEx 1.0, and confidence intervals surrounding each threshold remained wide. This was mostly likely due to the fact that the quality of studies published since the first iteration of ToMEx has not substantially improved. Given the effort required to update the ToMEx database, it is suggested that future updates only incorporate studies and data that meet strict screening criteria to ensure data utility. Despite this, ToMEx remains a useful tool for the research community, and this exercise demonstrates that large, coordinated data-mining efforts are feasible.