Publications : 2000

Mcnamara JF, Lombardo H, Pillai SK, Jensen I, Albericio F, Kates SK. 2000. An efficient solid-phase strategy for the construction of chemokines. J Pept Sci 6(10):512–518; doi: 10.1002/1099-1387(200010)6:10<512::AID-PSC269>3.0.CO;2-A.

Abstract

Synthesis of chemokines via stepwise SPPS approaches has been shown to be a challenge. Herein, a complete study of different coupling methods, solvents and temperature combined with a continuous-flow synthesizer equipped with feedback monitoring was carried out. The results from this study indicate that this family of molecules can be prepared using an Fmoc/But chemical approach and provide a general method to apply for the elongation of other difficult sequences.