The adoption of evidence-based methodologies in the field of toxicology and environmental health has experienced rapid growth in the past decade. This demonstrates the advantages that systematic review processes (e.g., transparency, reproducibility, rigor) bring to an assessment process but also highlights challenges the field faces in applying evidence-based medicine techniques to toxicology. Success in advancing methodologies has been achieved via collaborative efforts to share experiences and develop, test, and refine tools and approaches, as well as provide educational opportunities. This session aims to summarize and provide case examples of the advancements and challenges in evidence-based methods in toxicology. It also will serve as a forum for participants to learn about the direction of evidence-based methods, collaboration opportunities, and educational opportunities. This session will open with a brief introductory talk to characterize the scope of advances made in evidence-based toxicology over the past decade. A series of presentations will then highlight application of these methods to investigations of safety of food, drugs, and chemicals. A concluding talk will summarize future directions and challenges the toxicology community faces and its vision for the future. An open comment period will allow for identification and discussion of ongoing collaboration efforts open to the field.